Recombinant Mouse Negr1 protein(Met1-Gly318), His-tagged

• Cat.No.: Negr1-3801M
• Product Overview: Recombinant Mouse NEGR1 (NP_001034183.1) (Met 1-Gly 318) without the pro peptide was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gly318
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant mouse NEGR1 comprises 298 amino acids and has a calculated molecular mass of 33 kDa. As a result of glycosylation, rmNEGR1 migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Negr1 neuronal growth regulator 1 [ Mus musculus ]
• Official Symbol: Negr1
• Synonyms: NEGR1; neuronal growth regulator 1; neurotractin; kindred of IgLON; Ntra; KILON; mKIAA3001; 5330422G01Rik
• Gene ID: 320840
• mRNA Refseq: NM_001039094
• Protein Refseq: NP_001034183