Recombinant Mouse Musk Protein, His-tagged

• Cat.No.: Musk-42M
• Product Overview: Human embryonic kidney cell, HEK293-derived mouse MuSK protein
  Glu22-Thr494, with a C-terminal 6-His tag

Specification

• Description: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: HEK293
• Species: Mouse
• Tag: C-6×His
• Protein length: 22-494aa
• Bio-activity: Measured by its binding ability in a functional ELISA. Recombinant Mouse MuSK His-tag binds to Recombinant Mouse LRP-4 His-tag and Agrin His-tag with a ED50 of 0.0800-0.800 μg/mL.
• Molecular Mass: 53 kDa
• Endotoxin: < 0.1 EU/μg by LAL.
• N-terminal Sequence Analysis: Glu22
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
• Shipping: The product is shipped with dry ice or equivalent.
• References: 1. Valenzuela, D.M. et al. (1995) Neuron 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.

Gene Information

• Gene Name: Musk muscle, skeletal, receptor tyrosine kinase [ Mus musculus (house mouse) ]
• Official Symbol: Musk
• Synonyms: MUSK; muscle, skeletal, receptor tyrosine kinase; muscle, skeletal receptor tyrosine-protein kinase; muscle localized kinase 2; muscle-specific kinase receptor; muscle-specific protein kinase secretory isoform; muscle-specific tyrosine protein kinase receptor; muscle-specific tyrosine-protein kinase receptor; muscle, skeletal receptor tyrosine protein kinase; Mlk; Mdk4; Nsk1; Nsk2; Nsk3; MGC117677
• Gene ID: 18198
• mRNA Refseq: NM_001037127
• Protein Refseq: NP_001032204
• UniProt ID: Q61006