Recombinant Mouse Musk Protein, mIgG2a tagged

• Cat.No.: Musk-45M
• Product Overview: Recombinant Mouse Musk Protein (Glu22-Thr494) with Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Description: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: Mouse myeloma cell line
• Species: Mouse
• Tag: mIgG2a
• Protein length: 22-494aa
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. In the presence of Recombinant Mouse Agrin, immobilized Recombinant Mouse MuSK Fc Chimera (2 μg/mL at 100 μL/well) binds Recombinant Mouse LRP-4 with an ED50 of 150-900 ng/mL
• Molecular Mass: 79 kDa
• Endotoxin: < 0.1 EU/μg by LAL.
• Purity: > 95 %, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• N-terminal Sequence Analysis: Glu22
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  2 weeks, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• References: 1. Valenzuela, D.M. et al. (1995) Neuron 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.

Gene Information

• Gene Name: Musk muscle, skeletal, receptor tyrosine kinase [ Mus musculus (house mouse) ]
• Official Symbol: Musk
• Synonyms: MUSK; muscle, skeletal, receptor tyrosine kinase; muscle, skeletal receptor tyrosine-protein kinase; muscle localized kinase 2; muscle-specific kinase receptor; muscle-specific protein kinase secretory isoform; muscle-specific tyrosine protein kinase receptor; muscle-specific tyrosine-protein kinase receptor; muscle, skeletal receptor tyrosine protein kinase; Mlk; Mdk4; Nsk1; Nsk2; Nsk3; MGC117677
• Gene ID: 18198
• mRNA Refseq: NM_001037127
• Protein Refseq: NP_001032204
• UniProt ID: Q61006