Recombinant Mouse Mkln1 Protein, Myc/DDK-tagged

• Cat.No.: Mkln1-4082M
• Product Overview: Purified recombinant protein of mouse full-length muskelin 1, intracellular mediator containing kelch motifs (Mkln1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent GABRA1 degradation. Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1.
• Molecular Mass: 84.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mkln1 muskelin 1, intracellular mediator containing kelch motifs [ Mus musculus (house mouse) ]
• Official Symbol: Mkln1
• Synonyms: MKLN1; muskelin 1, intracellular mediator containing kelch motifs; muskelin; AU015903; BB154892; A130067F06Rik
• Gene ID: 27418
• mRNA Refseq: NM_013791
• Protein Refseq: NP_038819
• UniProt ID: O89050