Recombinant Mouse Mif, His-tagged

• Cat.No.: Mif-71M
• Product Overview: Recombinant mouse Mif, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Mif also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
• Form: Liquid. In Phosphate buffered saline containing 10% glycerol, 1mM DTT
• Molecular Mass: 14.9kDa (138aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPMFIVN TNVPRASVPE GFLSELTQQL AQATGKPAQY IAVHVVPDQL MTFSGTNDPC ALCSLHSIGK IGGAQNRNYS KLLCGLLSDR LHISPDRVYI NYYDMNAANV GWNGSTFA
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Mif macrophage migration inhibitory factor [ Mus musculus ]
• Official Symbol: Mif
• Synonyms: MIF; macrophage migration inhibitory factor; DER6; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; glycosylation-inhibiting factor; delayed early response protein 6; GIF; Glif; MGC107654
• Gene ID: 17319
• mRNA Refseq: NM_010798
• Protein Refseq: NP_034928
• Pathway: Adipogenesis, organism-specific biosystem; Phenylalanine metabolism, organism-specific biosystem; Phenylalanine metabolism, conserved biosystem; Tyrosine metabolism, organism-specific biosystem; Tyrosine metabolism, conserved biosystem
• Function: cell surface binding; chemoattractant activity; cytokine activity; cytokine activity; cytokine receptor binding; dopachrome isomerase activity; isomerase activity; phenylpyruvate tautomerase activity; phenylpyruvate tautomerase activity; protein complex binding; receptor binding