Recombinant Mouse Mgrn1 Protein, Myc/DDK-tagged

• Cat.No.: Mgrn1-4063M
• Product Overview: Purified recombinant protein of mouse full-length mahogunin, ring finger 1 (Mgrn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: E3 ubiquitin-protein ligase. Mediates TSG101 monoubiquitination at multiple sites. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling.
• Molecular Mass: 58.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mgrn1 mahogunin, ring finger 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mgrn1
• Synonyms: MGRN1; mahogunin, ring finger 1; E3 ubiquitin-protein ligase MGRN1; mahoganoid; mahogunin RING finger protein 1; probable E3 ubiquitin-protein ligase MGRN1; md; nc; mKIAA0544; 2610042J20Rik
• Gene ID: 17237
• mRNA Refseq: NM_029657
• Protein Refseq: NP_083933
• UniProt ID: Q9D074