Recombinant Mouse Mep1b Protein, His-tagged

• Cat.No.: Mep1b-43M
• Product Overview: Recombinant mouse MEP1B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: MEP1B, also known as meprin A subunit beta, is a member of the astacin family of zinc endopeptidases 1, 2. It is disulfide-linked, tetrameric metalloendopeptidases in renal brush border membranes. It is highly regulated, secreted, and cell-surface metalloendopeptidases that are abundantly expressed in the kidney and intestine.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 72.6kDa (642aa)70-100kDa (SDS-PAGE under reducing conditions)
• AA Sequence: LPAPEKFVKD IDGGIDQDIF DINQGLGLDL FEGDIKLEAN GKNSIIGDHK RWPHTIPYVL EDSLEMNAKG VILNAFERYR LKTCIDFKPWSGEANYISVF KGSGCWSSVG NIHAGKQELS IGTNCDRIAT VQHEFLHALG FWHEQSRADR DDYVIIVWDR IQPGKEHNFN IYNDSVSDSLNVPYDYTSVM HYSKTAFQNG TESTIVTRIS EFEDVIGQRM DFSDYDLLKL NQLYNCTSSL SFMDSCDFEL ENICGMIQSS GDSADWQRVSQVLSGPESDH SKMGQCKDSG FFMHFNTSIL NEGATAMLES RLLYPKRGFQ CLEFYLYNSG SGNDQLNIYT REYTTGQQGG VLTLQRQIKEVPIGSWQLHY VTLQVTKKFR VVFEGLRGPG TSSGGLSIDD INLSETRCPH HIWHIQNFTQ ILGGQDTSVY SPPFYSSKGY AFQIYMDLRSSTNVGIYFHL ISGANDDQLQ WPCPWQQATM TLLDQNPDIR QRMFNQRSIT TDPTMTSDNG SYFWDRPSKV GVTDVFPNGT QFSRGIGYGTTVFITRERLK SREFIKGDDI YILLTVEDIS HLNSTSAVPD PVPTLAVHNA CSEVVCQNGG ICVVQDGRAE CKCPAGEDWW YMGKRCEKRGSTRDVEHHHH HH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Mep1b meprin 1 beta [ Mus musculus ]
• Official Symbol: Mep1b
• Synonyms: MEP1B; meprin 1 beta; meprin B; meprin beta; Mep-1b; MGC159330
• Gene ID: 17288
• mRNA Refseq: NM_008586
• Protein Refseq: NP_032612
• Pathway: Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem
• Function: hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding