Recombinant Mouse Med8 Protein, Myc/DDK-tagged

• Cat.No.: Med8-4022M
• Product Overview: Purified recombinant protein of mouse full-length mediator complex subunit 8 (Med8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
• Molecular Mass: 29.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Med8 mediator complex subunit 8 [ Mus musculus (house mouse) ]
• Official Symbol: Med8
• Synonyms: MED8; mediator of RNA polymerase II transcription, subunit 8 homolog (yeast); mediator of RNA polymerase II transcription subunit 8; mediator complex subunit 8; activator-recruited cofactor 32 kDa component; ARC32; AB041805; MNCb-2386; 2210021A15Rik
• Gene ID: 80509
• mRNA Refseq: NM_020000
• Protein Refseq: NP_064384
• UniProt ID: Q9D7W5