Recombinant Mouse Mastl Protein, Myc/DDK-tagged

• Cat.No.: Mastl-3968M
• Product Overview: Purified recombinant protein of mouse full-length microtubule associated serine/threonine kinase-like (Mastl), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited.
• Molecular Mass: 96.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mastl microtubule associated serine/threonine kinase-like [ Mus musculus (house mouse) ]
• Official Symbol: Mastl
• Synonyms: MASTL; microtubule associated serine/threonine kinase-like; serine/threonine-protein kinase greatwall; microtubule-associated serine/threonine-protein kinase-like; GW; GWL; THC2; C88295; MAST-L; 2700091H24Rik; MGC117975
• Gene ID: 67121
• mRNA Refseq: NM_025979
• Protein Refseq: NP_080255
• UniProt ID: Q8C0P0