Recombinant Mouse Mapk1 Protein, Gly/Pro-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Mapk1-482MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Mapk1 (P63085) (Met1-Ser358), fused with two additional amino acids (Gly & Pro) at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Species: Mouse
• Source: Insect Cells
• ProteinLength: 360
• Form: Lyophilized
• Molecular Mass: The recombinant mouse MAPK1 consists of 360 amino acids and predicts a molecular mass of 41.4 kDa. It migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Gly
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Mapk1 mitogen-activated protein kinase 1 [ Mus musculus ]
• Official Symbol: Mapk1
• Gene ID: 26413
• mRNA Refseq: NM_001038663
• Protein Refseq: NP_001033752