Recombinant Mouse Map4k2 Protein, Myc/DDK-tagged

• Cat.No.: Map4k2-3933M
• Product Overview: Purified recombinant protein of mouse full-length mitogen-activated protein kinase kinase kinase kinase 2 (Map4k2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 91.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Map4k2 mitogen-activated protein kinase kinase kinase kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Map4k2
• Synonyms: MAP4K2; mitogen-activated protein kinase kinase kinase kinase 2; MEKKK 2; MEK kinase kinase 2; germinal center kinase; RAB8 interacting protein; rab8-interacting protein; MAPK/ERK kinase kinase kinase 2; mitogen activated protein kinase kinase kinase kinase 2; GCK; BL44; Rab8ip; AI385662; MGC143893; MGC143894
• Gene ID: 26412
• mRNA Refseq: NM_009006
• Protein Refseq: NP_033032
• UniProt ID: Q61161