Recombinant Mouse Lipopolysaccharide Binding Protein

Cat. No. :	Lbp-247M
Description :	LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off. up to 2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (2 x 106/ml).
Source :	CHO-cells.
Application And Suggested Dilutions :	ELISA, Inhibition Assays, Western Blotting.
Formulation :	Recombinant Mouse LBP was lyophilized from a protein solution (0.5 mg/ml) containing phosphate-buffered saline, pH 7.2.
Characterization :	On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa.
Storage & Stability :	Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.
Tag :	Non
Full Length :	Full L.

Gene Name	Lbp lipopolysaccharide binding protein [ Mus musculus ]
Synonyms	Lbp; lipopolysaccharide binding protein; Ly88
Gene ID	16803
mRNA Refseq	NM_008489
Protein Refseq	NP_032515
UniProt ID	Q61805
Chromosome Location	2 H1; 2 83.0 cM
Pathway	Toll-like receptor signaling pathway
Function	lipid binding; lipopolysaccharide binding; lipoteichoic acid binding; receptor binding