Recombinant Human Lipopolysaccharide Binding Protein, His-tagged

• Cat.No.: LBP-4927H
• Product Overview: Recombinant human LBP containing His tag was expressed by baculovirus in CHO cells and having a molecular mass of 58.0 kDa.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Description: Natural LBP is a 58 kDa glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophils and granulocytes. This is a His-tagged protein
• Biological Activity: Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS).
• Molecular Weight: 58.0 kDa
• Form: Lyophilized from a concentrated protein solution (0.25mg/ml) containing PBS, pH 7.2
• Endotoxin Level: < 0.03 ng/ ml
• Appearance: Lyophilized protein
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in 40 µl of ultra-pure distilled water. This solution can then be diluted into other aqueous buffers and stored at -20°C or -70°C for future use.
• Purity: >90-95% by SDS-PAGE analyses
• Storage: The lyophilized protein is best-stored desiccated below 0°C. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% BSA). Repeated freezing and thawing should be avoided.
• Pathways: Toll-like receptor signaling pathway; Signaling in Immune system

Gene Information

• Gene Name: LBP lipopolysaccharide binding protein [ Homo sapiens ]
• Official Symbol: LBP
• Synonyms: LBP; lipopolysaccharide binding protein; BPIFD2; MGC22233; lipopolysaccharide-binding protein; OTTHUMP00000030965; LPS-binding protein; BPI fold containing family D, member 2
• Gene ID: 3929
• mRNA Refseq: NM_004139
• Protein Refseq: NP_004130
• MIM: 151990
• UniProt ID: P18428
• Chromosome Location: 20q11.23-q12
• Function: Gram-negative bacterial cell surface binding; Gram-positive bacterial cell surface binding; cell surface binding; lipid binding; lipopolysaccharide binding; lipoteichoic acid binding; receptor binding