Recombinant Mouse LGMN Protein, His-tagged

• Cat.No.: LGMN-31M
• Product Overview: Recombinant mouse LGMN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: LGMN, also known as legumain, is a lysosomal cysteine protease found in all mouse tissues, but was particularly abundant in kidney and placenta. It plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases. Over expression of Legumain in tumors is significant for invasion/metastasis.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 48.6kDa (426aa)40-57KDa (SDS–PAGE under reducing conditions)
• AA Sequence: VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTGEDVTPENFLA VLRGDAEAVK GKGSGKVLKS GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMMNHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQGMKHRASSPIS LPPVTHLDLT PSPDVPLTIL KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTAHDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM DKVCLSHYLE HHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS – PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Lgmn legumain [ Mus musculus ]
• Official Symbol: LGMN
• Synonyms: LGMN; legumain; preprolegumain; protease, cysteine 1; protease, cysteine, 1; asparaginyl endopeptidase; AEP; Prsc1; AI746452; AU022324
• Gene ID: 19141
• mRNA Refseq: NM_011175
• Protein Refseq: NP_035305
• Pathway: Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Metabolism of steroid hormones and vitamins A and D, organism-specific biosystem
• Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; peptidase activity; peptidase activity