Recombinant Mouse Lefty2 protein, His-tagged

• Cat.No.: Lefty2-7973M
• Product Overview: Recombinant Mouse Lefty2 aa. (Tyr247~Asp358 (Accession # P57785)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Tyr247~Asp358
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This protein is also important in self-renewal and differentiation of mouse embryonic stem cells. Mice lacking a functional copy of this gene exhibit defects in axial and left-right patterning.
• Form: Freeze-dried powder
• Molecular Mass: 14 kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.6.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Lefty2 left-right determination factor 2 [ Mus musculus (house mouse) ]
• Official Symbol: Lefty2
• Synonyms: Lefty2; left-right determination factor 2; Ebaf; Lefta; Leftb; lefty-2; lefty-B; 6030463A22Rik; left-right determination factor 2; endometrial bleeding associated factor; left-right determination factor B; left-right determination, factor A
• Gene ID: 320202
• mRNA Refseq: NM_177099.4
• Protein Refseq: NP_796073.1
• UniProt ID: P57785