Recombinant Mouse Kdm5b, GST-tagged

Description :	KDM5B is a lysine (K)-specific demethylase 5B which is highly expressed in testis and shows the lowest expression in skeletal muscle. KDM5B is a histone H3 lysine 4 (H3K4) demethylase up-regulated in prostate cancer and associated with androgen receptor where it regulated its transcriptional activity in reporter gene assays. Overexpression of KDM5B in HeLa cells results in loss of tri-, di-, and monomethyl H3K4, but does not alter the methylation status of H3K9 or H3K36. A KDM5B containing highly conserved putative DNA/chromatin binding motif is specifically up-regulated in breast cancer.
Source :	Sf9 insect cells using baculovirus
Species :	Mouse
Tag :	GST
Form :	Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Molecular Mass :	~112 kDa
Purity :	>90% by densitometry
Applications :	Western Blot
Storage :	Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration :	0.1 μg/μl
Protein length :	1-753 a.a.

Gene Name	Kdm5b lysine (K)-specific demethylase 5B [ Mus musculus ]
Official Symbol	Kdm5b
Synonyms	KDM5B; lysine (K)-specific demethylase 5B; lysine-specific demethylase 5B; histone demethylase JARID1B; putative DNA/chromatin binding motif 1; jumonji/ARID domain-containing protein 1B; jumonji, AT rich interactive domain 1B (Rbp2 like); PUT1; Plu1; PLU-1; Rb-Bp2; Jarid1b; AW556288; RBBP2H1A; mKIAA4034; D1Ertd202e; 2010009J12Rik; 2210016I17Rik; KIAA4034;
Gene ID	75605
mRNA Refseq	NM_152895
Protein Refseq	NP_690855
Function	DNA binding; histone demethylase activity (H3-K4 specific); histone demethylase activity (H3-dimethyl-K4 specific); histone demethylase activity (H3-trimethyl-K4 specific); metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; transcription corepressor activity; zinc ion binding;