Recombinant Mouse Ipo4 Protein, Myc/DDK-tagged

• Cat.No.: Ipo4-3565M
• Product Overview: Purified recombinant protein of mouse full-length importin 4 (Ipo4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPS3A. Acts as chaperone for exposed basic domains.
• Molecular Mass: 119.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ipo4 importin 4 [ Mus musculus (house mouse) ]
• Official Symbol: Ipo4
• Synonyms: IPO4; importin 4; importin-4; imp4; importin 4a; importin-4a; ran-binding protein 4; Imp4a; RanBP4; AA409693; 8430408O15Rik; MGC113723
• Gene ID: 75751
• mRNA Refseq: NM_024267
• Protein Refseq: NP_077229
• UniProt ID: Q8VI75