Recombinant Mouse Interleukin 6

• Cat.No.: Il6-76M
• Product Overview: Recombinant Mouse Interleukin-6 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton. The IL-6 is purified by proprietary chromatographic techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1 mg/ml) solution containing 10 mM NaAcetate, pH 6.0.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin 6 in 20 mM HCl to 0.1-1.0 mg/ml to regain full activity, and can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.02 ng/ml, corresponding to a Specific Activity of 5 x 10⁷IU/mg.
• Stability: Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°Cbetween 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Il6 interleukin 6 [ Mus musculus ]
• Synonyms: Il6; interleukin 6; Il-6
• Gene ID: 16193
• mRNA Refseq: NM_031168
• Protein Refseq: NP_112445
• UniProt ID: P08505
• Chromosome Location: 5 B1; 5 17.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Graft-versus-host disease; Hematopoietic cell lineage; Hypertrophic cardiomyopathy (HCM); Jak-STAT signaling pathway; Pathways in cancer; Prion diseases; Toll-like receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-6 receptor binding; protein binding