Active Recombinant Mouse IL6 protein, His-tagged

• Cat.No.: IL6-0302M
• Product Overview: Recombinant Mouse IL6 protein(Phe25-Thr211), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 25-211 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Mouse IL-6, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-6 R alpha, His,Avitag with a linear range of 20-156 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 23.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe25-Thr211
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il6 interleukin 6 [ Mus musculus ]
• Official Symbol: IL6
• Synonyms: IL6; interleukin 6; interleukin-6; interleukin HP-1; B-cell hybridoma growth factor; Il-6
• Gene ID: 16193
• mRNA Refseq: NM_031168
• Protein Refseq: NP_112445