Recombinant Mouse Interleukin-22

• Cat.No.: Il22-28M
• Product Overview: Recombinant Mouse IL-22 produced in E. coli is a single, non-glycosylated polypeptide chain containing 147 amino acids and having a molecular mass of 16.7 kDa.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II cytokine receptor family.
• Purity: >97% as determined by RP-HPLC and SDS-PAGE.
• Formulation: rmIL-22 was lyophilized after extensive dialysis against PBS.
• Specific Activity: Recombinant Mouse IL-22 is fully biologically active when compared to standard. The ED50 as determined by its ability to induce IL-10 secretion in Colo205 cells is less than 0.5ng/ml, corresponding to a Specific Activity of 2.0 x 10⁶IU/mg.
• Endotoxin: Less than 0.1ng/µg (1 IEU/µg) determined by LAL test.
• Reconstitution: It is recommended to reconstitute the lyophilized rmIL-22 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized rmIL-22 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rmIL-22 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Il22
• Synonyms: IL-22, IL-TIF, ILTIF, TIFa, IL-10-related T-cell-derived-inducible factor, ILTIFA, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23, Interleukin-22, IL-TIF alpha, Interleukin-22a, IL-22a, Interleukin-22 Precursor
• Gene ID: 50929
• mRNA Refseq: NM_016971.1
• Protein Refseq: NP_058667.1
• MIM: 605330
• UniProt ID: Q9JJY9
• Chromosome Location: 10 67.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
• Function: cytokine activity; protein binding