Recombinant Human Interleukin 22, HQ-tagged

• Cat.No.: IL22-637H
• Product Overview: Recombinant humanIL22 contains 146 amino acids and a 16 aa HQ tag. Total length is 162 aa.

Specification

• Species: Human
• Source: Hordeum Vulgare
• Tag: HQ
• Description: IL22 is proteinthat in humans is encoded by the IL22 gene. IL22 a member of a group ofcytokines called the IL-10 family or IL-10 superfamily (including IL-19,IL-20, IL-24, and IL-26), a class of potent mediators of cellularinflammatory responses. It shares use of IL-10R2 in cell signaling with othermembers of this family, IL-10, IL-26, IL-28A/B and IL-29. IL22 is produced byactivated DC and T cells and initiates innate immune responses againstbacterial pathogens especially in epithelial cells such as respiratory andgut epithelial cells. IL22 along with IL-17 is rapidly produced by splenicLTi-like cells and can be also produced by Th17 cells and likely plays a rolein the coordinated response of both adaptive and innate immune systems.
• Form: Lyophilized from a0.2 µm sterile filtered solution of PBS, pH 7.2.
• Molecular Weight: 18.9 kDa includingHQ tag. The glycosylated IL22 migrates as 3 bands with an apparent MW = 22,24, 26 kDa in SDS-PAGE.
• Purity: >98% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction.
• Endotoxin Level: < 0.005 ng perµg of product (0.05 EU/µg) as measured by turbidimetric kinetic assay.
• Biological Activity: Bioactivity isassayed by measuring the dose dependent effect of human IL22 on proliferationof Ba/F3 cells transfected with mouse IL22 receptor. The ED50 for for a typicalbatch of recombinant human IL22 is 2.2 ng/ml.
• Reconstitution: Centrifuge vialprior to opening. Reconstitute the lyophilized protein in sterile water to aconcentration of no less than 100 µg/ml.
• Storage: The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: IL22

Gene Information

• Gene Name: IL22 interleukin 22 [ Homosapiens ]
• Synonyms: IL22; interleukin22; TIFa; IL-21; IL21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; ZCYTO18;MGC79382; MGC79384; TIFIL-23; interleukin-22; interleukin 21; cytokineZcyto18; Cytokine Zcyto18; OTTHUMP00000240117; IL-10-related T-cell-derivedinducible factor; IL-10-related T-cell-derived-inducible factor
• Gene ID: 50616
• mRNA Refseq: NM_020525
• Protein Refseq: NP_065386
• MIM: 605330
• UniProt ID: Q9GZX6
• Chromosome Location: 12q15
• Pathway: Cytokine-cytokinereceptor interaction; Jak-STAT signaling pathway
• Function: cytokine activity;interleukin-22 receptor binding