Recombinant Mouse Il5 protein(Met1-Gly133), His-tagged

• Cat.No.: Il5-755M
• Product Overview: Recombinant Mouse IL5 (P04401) (Met 1-Gly 133) was expressed in Insect Cells, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: 1-133 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% gly. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.
• Molecular Mass: The recombinant mouse IL5 consists of 123 amino acids and has a calculated molecular mass of 14.5 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions, and the homodimer form could also be seen.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il5 interleukin 5 [ Mus musculus ]
• Official Symbol: Il5
• Synonyms: IL5; interleukin 5; interleukin-5; TRF; BCGF-II; B-cell growth factor II; T-cell replacing factor; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor; Il-5
• Gene ID: 16191
• mRNA Refseq: NM_010558
• Protein Refseq: NP_034688