Recombinant Mouse Il1b protein, His-tagged

• Cat.No.: Il1b-509M
• Product Overview: Recombinant Mouse Il1b (P10749) aa. (Val118~Ser269) fused with His tag was produced in HEK293F cells.

Specification

• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response.
• Source: HEK293F
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19kDa; Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Val118~Ser269
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 97%
• Characteristic: Isoelectric point is 8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Il1b interleukin 1 beta [ Mus musculus (house mouse) ]
• Official Symbol: Il1b
• Synonyms: Il1b; interleukin 1 beta; Il-1b; IL-1beta; interleukin-1 beta
• Gene ID: 16176
• mRNA Refseq: NM_008361.4
• Protein Refseq: NP_032387.1
• UniProt ID: P10749