Recombinant Mouse Il12b protein(Met1-Ser335), hFc-tagged

• Cat.No.: Il12b-738M
• Product Overview: Recombinant Mouse IL12B (P43432) (Met 1-Ser 335) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 1-335 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse IL12B-Fc at 10μg/mL (100μL/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 0.10-0.24μg/mL.
• Molecular Mass: The secreted recombinant mouse IL12B/Fc is a disulfide-linked homodimer. The reduced monomer comprises 554 amino acids and has a calculated molecular mass of 62.8 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 83 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il12b interleukin 12b [ Mus musculus ]
• Official Symbol: Il12b
• Synonyms: IL12B; interleukin 12b; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; p40; Il-12b; Il12p40; Il-12p40
• Gene ID: 16160
• mRNA Refseq: NM_008352
• Protein Refseq: NP_032378