Recombinant Human IL12B
Cat.No. : | IL12B-220H |
Product Overview : | Recombinant Human interleukin 12B DNA sequences encoding the p40 subunit of the human IL-12 protein was expressed inSf 21 insect cells. |
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Species : | Human |
Source : | Sf21 Cells |
Tag : | Non |
Description : | Interleukin 12 (IL-12, NK cell stimulatory factor, cytotoxic lymphocyte maturation factor) is a heterodimeric cytokine that is naturally produced by dendritic cells , macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th1 cells, which is important in resistance against pathogens. It is known as a T cell stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity, which can block the formation of new blood vessels. IL-12 binds to the IL-12 receptor and upon binding, IL-12R-β2 becomes tyrosine phosphorylated and provides binding sites for kinases, Tyk2 and Jak2. These are important in activating critical transcription factor proteins such as STAT4 which are implicated in IL-12 signaling in T cells and NK cells. |
Molecular Weight : | The predicted molecular weight of Recombinant Human IL-12 is Mr 40 kDa. |
State Of Matter : | Lyophilized. |
Formulation : | This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 μg of bovine serum albumin per 1 μg of cytokine with no calcium, magnesium, or preservatives. |
Purity : | > 90% by SDS Page and analyzed by silver stain. |
Endotoxin : | < 1.0 EU/µg as determined by the LAL method. |
Biological Activity : | The monomeric human IL-12 p40 monomer exhibits no IL-12 antagonist activity. rhIL-12 p40 is intended for use as a control for anti-human IL-12 p40 antibodies. |
Storage And Stability : | This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |
Gene Name | IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ] |
Synonyms | OTTHUMP00000160820; CLMF; CLMF2; IL-12B; NKSF; NKSF2; CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; IL12, subunit p40; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); IL12B |
Gene ID | 3593 |
mRNA Refseq | NM_002187 |
Protein Refseq | NP_002178 |
UniProt ID | P29460 |
Chromosome Location | 5q31.1-q33.1 |
MIM | 161561 |
Pathway | Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus |
Function | contributes_to cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-12 alpha subunit binding; interleukin-12 receptor binding; protein binding; protein homodimerization activity; signal transducer activity |
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Not For Human Consumption!
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