Recombinant Mouse Igf1 protein, His-tagged

• Cat.No.: Igf1-811M
• Product Overview: Recombinant Mouse Igf1 aa. (Gly33~Ala102 (Accession # Q8CAR0)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Gly33~Ala102
• Description: This gene encodes a member of the insulin-like growth factor (IGF) family of proteins that promote growth and development during fetal and postnatal life. This gene is predominantly expressed in the liver and the encoded protein undergoes proteolytic processing to generate a disulfide-linked mature polypeptide. Transgenic disruption of this gene in mice results in reduced postnatal survival and severe growth retardation. Mice lacking the encoded protein exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
• Form: Freeze-dried powder
• Molecular Mass: 9.2 kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH 7.4.
• Reconstitution: Reconstitute in PBS.

Gene Information

• Gene Name: Igf1 insulin-like growth factor 1 [ Mus musculus (house mouse) ]
• Official Symbol: Igf1
• Synonyms: Igf1; insulin-like growth factor 1; Igf-1; Igf-I; C730016P09Rik; insulin-like growth factor I; somatomedin
• Gene ID: 16000
• mRNA Refseq: NM_001111274.1
• Protein Refseq: NP_001104744.1
• UniProt ID: Q8CAR0