Active Recombinant Human IGF1 protein (Gly49-Ala118(Glu 51 Arg))

• Cat.No.: IGF1-55H
• Product Overview: Recombinant human IGF1 (P05019-1) (Gly49-Ala118, with mutation Glu 51 Arg) protein, fused with a 13 amino acids (MFPAMPLSSLFVN) at the N-terminus, was expressed in HEK293 Cells.

Specification

• Species: Human
• Source: Yeast
• Protein Length: 49-118 a.a.
• CommonName: IGF1
• Predicted N Terminal: Met
• Form: Lyophilized from sterile PBS, pH7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 1.5-6 ng/mL.
• Molecular Mass: The recombinant human IGF1 consists of 83 amino acids and predicts a molecular mass of 9.1 kDa.
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: IGF1 insulin-like growth factor 1 (somatomedin C) [ Homo sapiens ]
• Official Symbol: IGF1
• Synonyms: IGF1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; IGF1A; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; IGFI; IGF-I
• Gene ID: 3479
• mRNA Refseq: NM_000618
• Protein Refseq: NP_000609
• MIM: 147440
• UniProt ID: P05019