Recombinant Mouse Ifng protein, His-tagged

• Cat.No.: Ifng-532M
• Product Overview: Recombinant Mouse Ifng (P01580) aa. (His23~Cys155) fused with His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: His23~Cys155
• Description: This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.8kDa; Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 8.8.
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Ifng interferon gamma [ Mus musculus (house mouse) ]
• Official Symbol: Ifng
• Synonyms: Ifng; interferon gamma; Ifg; IFN-g; IFN-gamma; gamma interferon
• Gene ID: 15978
• mRNA Refseq: NM_008337.4
• Protein Refseq: NP_032363.1
• UniProt ID: P01580