Recombinant Mouse Ifna14 protein(Cys24-Lys189), His-tagged

• Cat.No.: Ifna14-61M
• Product Overview: Recombinant Mouse IFNA14 (NP_996858.1)(Cys24-Lys189) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: 24-189 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 5-40 pg/mL.
• Molecular Mass: The recombinant mouse IFNA14 comprises 177 amino acids and has a predicted molecular mass of 20.5 kDa. The apparent molecular mass of the protein is approximately 20 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ifna14 interferon, alpha 14 [ Mus musculus ]
• Official Symbol: Ifna14
• Synonyms: IFNA14; interferon, alpha 14; RP23-67C1.2; MGC155959; OTTMUSG00000007662
• Gene ID: 404549
• mRNA Refseq: NM_206975
• Protein Refseq: NP_996858