Recombinant Mouse Idh3a Protein, Myc/DDK-tagged

• Cat.No.: Idh3a-3473M
• Product Overview: Purified recombinant protein of mouse full-length isocitrate dehydrogenase 3 (NAD+) alpha (cDNA clone MGC:19310 IMAGE:4166193), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
• Molecular Mass: 31.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Idh3a isocitrate dehydrogenase 3 (NAD+) alpha [ Mus musculus (house mouse) ]
• Official Symbol: Idh3a
• Synonyms: Idh3a; isocitrate dehydrogenase 3 (NAD+) alpha; AA407078; AI316514; 1110003P10Rik; 1500012E04Rik; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; NAD(+)-specific ICDH subunit alpha; isocitric dehydrogenase subunit alpha; EC 1.1.1.41
• Gene ID: 67834
• mRNA Refseq: NM_029573
• Protein Refseq: NP_083849
• UniProt ID: Q9D6R2