Recombinant Mouse Hif1an Protein, Myc/DDK-tagged

• Cat.No.: Hif1an-3394M
• Product Overview: Purified recombinant protein of mouse full-length hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Hydroxylates HIF-1 alpha at 'Asn-799' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 40.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Hif1an hypoxia-inducible factor 1, alpha subunit inhibitor [ Mus musculus (house mouse) ]
• Official Symbol: Hif1an
• Synonyms: HIF1AN; hypoxia-inducible factor 1, alpha subunit inhibitor; hypoxia-inducible factor 1-alpha inhibitor; hypoxia-inducible factor asparagine hydroxylase; FIH1; 2310046M24Rik; A830014H24Rik
• Gene ID: 319594
• mRNA Refseq: NM_176958
• Protein Refseq: NP_795932
• UniProt ID: Q8BLR9