Recombinant Mouse Hcrt protein, His & GST-tagged

• Cat.No.: Hcrt-2651M
• Product Overview: Recombinant Mouse Hcrt aa. (Gln33~Pro124 (Accession # O55241)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors Hcrtr1 and Hcrtr2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 42.0kDa
• Protein length: Gln33~Pro124
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.2.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: Hcrt hypocretin [ Mus musculus (house mouse) ]
• Official Symbol: Hcrt
• Synonyms: Hcrt; hypocretin; PPOX; orexin; orexin A; orexin B; prepro-orexin
• Gene ID: 15171
• mRNA Refseq: NM_010410.2
• Protein Refseq: NP_034540.1
• UniProt ID: O55241