Recombinant Human HCRT Protein, His&SUMO-tagged

• Cat.No.: HCRT-1168H
• Product Overview: Recombinant Human HCRT Protein (Gln34-Met97) with His&SUMO tag was expressed in E. coli.

Specification

• Description: This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
• Source: E. coli
• Species: Human
• Tag: His&SUMO
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.5 kDa; Accurate Molecular Mass: 21 kDa
• Protein length: Gln34-Met97
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HCRT hypocretin neuropeptide precursor [ Homo sapiens (human) ]
• Official Symbol: HCRT
• Synonyms: HCRT; hypocretin neuropeptide precursor; OX; PPOX; NRCLP1; orexin; hypocretin (orexin) neuropeptide; prepro-orexin
• Gene ID: 3060
• mRNA Refseq: NM_001524
• Protein Refseq: NP_001515
• MIM: 602358
• UniProt ID: O43612