Recombinant Mouse Hck protein, His & T7-tagged

• Cat.No.: Hck-7855M
• Product Overview: Recombinant Mouse Hck aa. (Gly2~Pro524 (Accession # P08103)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.
• Source: E. coli
• Species: Mouse
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 62.7kDa.
• Protein length: Gly2~Pro524
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Hck hemopoietic cell kinase [ Mus musculus (house mouse) ]
• Official Symbol: Hck
• Synonyms: Hck; hemopoietic cell kinase; Bmk; AI849071; tyrosine-protein kinase HCK; B cell/myeloid kinase; hematopoietic cell kinase; p56-HCK/p59-HCK
• Gene ID: 15162
• mRNA Refseq: NM_001172117.1
• Protein Refseq: NP_001165588.1
• UniProt ID: P08103