Recombinant Mouse Gsdmd Protein, His-tagged

• Cat.No.: Gsdmd-1600M
• Product Overview: Recombinant Mouse Gsdmd Protein (Met1-Gln325) with N-His tag was expressed in E. coli.

Specification

• Description: Enables phospholipid binding activity and wide pore channel activity. Involved in several processes, including defense response to bacterium; positive regulation of interleukin-1 beta production; and protein-containing complex assembly. Acts upstream of or within cellular response to extracellular stimulus. Located in cytosol; extracellular space; and plasma membrane. Part of NLRP3 inflammasome complex. Is expressed in several structures, including gut; liver; lung; metanephros; and spleen. Orthologous to human GSDMD (gasdermin D).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 39.5 kDa
  Accurate Molecular Mass: 40 kDa
• Protein length: Met1-Gln325
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gsdmd gasdermin D [ Mus musculus (house mouse) ]
• Official Symbol: Gsdmd
• Synonyms: GSDMD; gasdermin D; gasdermin-D; gasdermin domain containing 1; gasdermin domain-containing protein 1; DF5L; M2-4; Dfna5l; Gsdmdc1; AW558049; 1810036L03Rik
• Gene ID: 69146
• mRNA Refseq: NM_026960
• Protein Refseq: NP_081236
• UniProt ID: Q9D8T2