Recombinant Mouse Foxa2 Protein, His-tagged

• Cat.No.: Foxa2-1510M
• Product Overview: Recombinant Mouse Foxa2 Protein (Met1-Ser459) with N-His tag was expressed in E. coli.

Specification

• Description: Enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and chromatin binding activity. Involved in several processes, including negative regulation of detection of glucose; negative regulation of glucokinase activity; and regulation of transcription, DNA-templated. Acts upstream of or within several processes, including animal organ development; chordate embryonic development; and regulation of neuron differentiation. Located in nucleus. Part of transcription regulator complex. Is expressed in several structures, including central nervous system; endoderm; genitourinary system; gut; and respiratory system. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human FOXA2 (forkhead box A2).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 52.2 kDa
  Accurate Molecular Mass: 53 kDa
• Protein length: Met1-Ser459
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Foxa2 forkhead box A2 [ Mus musculus (house mouse) ]
• Official Symbol: Foxa2
• Synonyms: FOXA2; forkhead box A2; hepatocyte nuclear factor 3-beta; HNF-3-beta; forkhead box protein A2; hepatocyte nuclear factor-3 beta; hepatocyte nuclear factor 3 beta (winged helix transcription factor); Hnf3b; Tcf3b; Hnf-3b; Tcf-3b; HNF3beta; HNF3-beta
• Gene ID: 15376
• mRNA Refseq: NM_010446
• Protein Refseq: NP_034576
• UniProt ID: P35583