Recombinant Mouse Eif4b Protein, Myc/DDK-tagged

• Cat.No.: Eif4b-2786M
• Product Overview: Purified recombinant protein of mouse full-length eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
• Molecular Mass: 40.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Eif4b eukaryotic translation initiation factor 4B [ Mus musculus (house mouse) ]
• Official Symbol: Eif4b
• Synonyms: Eif4b; eukaryotic translation initiation factor 4B; C85189; Eif4a2; AL024095; 2310046H11Rik; eukaryotic translation initiation factor 4B; eIF-4B
• Gene ID: 75705
• mRNA Refseq: NM_145625
• Protein Refseq: NP_663600
• UniProt ID: Q8BGD9