Recombinant Mouse Eif3d Protein, Myc/DDK-tagged

• Cat.No.: Eif3d-2778M
• Product Overview: Purified recombinant protein of mouse full-length eukaryotic translation initiation factor 3, subunit D (Eif3d), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs.
• Molecular Mass: 64 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Eif3d eukaryotic translation initiation factor 3, subunit D [ Mus musculus (house mouse) ]
• Official Symbol: Eif3d
• Synonyms: EIF3D; eukaryotic translation initiation factor 3, subunit D; eukaryotic translation initiation factor 3 subunit D; eIF3 p66; eIF-3-zeta; translation initiation factor eIF3 p66; eukaryotic translation initiation factor 3 subunit 7; eukaryotic translation initiation factor 3, subunit 7 (zeta); Eif3s7; eIF3p66; 66/67kDa; AA407891; MGC115774
• Gene ID: 55944
• mRNA Refseq: NM_018749
• Protein Refseq: NP_061219
• UniProt ID: O70194