Recombinant Mouse Dusp28 Protein, Myc/DDK-tagged

• Cat.No.: Dusp28-2688M
• Product Overview: Purified recombinant protein of mouse full-length dual specificity phosphatase 28 (Dusp28), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate (in vitro). Has almost no detectable activity with phosphotyrosine, even less activity with phosphothreonine and displays complete lack of activity with phosphoserine. The poor activity with phosphotyrosine may be due to steric hindrance by bulky amino acid sidechains that obstruct access to the active site.
• Molecular Mass: 17.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dusp28 dual specificity phosphatase 28 [ Mus musculus (house mouse) ]
• Official Symbol: Dusp28
• Synonyms: DUSP28; dual specificity phosphatase 28; AV005521; 0710001B24Rik
• Gene ID: 67446
• mRNA Refseq: NM_175118
• Protein Refseq: NP_780327
• UniProt ID: Q8BTR5