Recombinant Mouse Dmpk Protein, His-tagged

• Cat.No.: Dmpk-1353M
• Product Overview: Recombinant Mouse Dmpk Protein (Met1-Lys109) with N-His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Met1-Lys109
• Description: The protein encoded by this gene is a serine/threonine protein kinase that contains coiled-coil and C-terminal membrane association domains. In the embryonic mouse, it is found in cardiac and skeletal myocytes where it appears to play a role in myogenesis. In adults, the transcript is localized to several tissues including brain, heart, and skeletal and smooth muscle, and a function in cytoskeletal remodeling has been described. Transcripts with expanded CUG repeats in the 3' untranslated region mediate alternative splicing of several genes and sequester RNA binding proteins and RNA transcripts that contain CAG repeats, resulting in myotonic dystrophy, an autosomal dominant neuromuscular disorder. Alternative splicing results in multiple protein coding and non-coding transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.1 kDa
  Accurate Molecular Mass: 16 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Dmpk dystrophia myotonica-protein kinase [ Mus musculus (house mouse) ]
• Official Symbol: Dmpk
• Synonyms: DMPK; dystrophia myotonica-protein kinase; myotonin-protein kinase; DM-kinase; dystrophia myotonica kinase, B15; myotonic dystrophy protein kinase; DM; DMK; Dm15; MDPK; MT-PK
• Gene ID: 13400
• mRNA Refseq: NM_001190490
• Protein Refseq: NP_001177419
• UniProt ID: P54265