Recombinant Mouse Dhfr Protein, Myc/DDK-tagged

• Cat.No.: Dhfr-2545M
• Product Overview: Purified recombinant protein of mouse full-length dihydrofolate reductase (Dhfr), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
• Molecular Mass: 21.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dhfr dihydrofolate reductase [ Mus musculus (house mouse) ]
• Official Symbol: Dhfr
• Synonyms: DHFR; dihydrofolate reductase; AA607882; AI662710; AW555094; 8430436I03Rik
• Gene ID: 13361
• mRNA Refseq: NM_010049
• Protein Refseq: NP_034179
• UniProt ID: P00375