Dhfr
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Official Full Name
dihydrofolate reductase -
Overview
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014] -
Synonyms
DHFR;dihydrofolate reductase;DYR;DHFRP1
Recombinant Proteins
- Human
- Chicken
- Mouse
- Zebrafish
- Hamster
- E.coli
- Mammalian Cells
- Wheat Germ
- HEK293
- In Vitro Cell Free System
- GST
- His
- Non
- Flag
- Avi&Fc&His
- DDK&Myc
- His&SUMO
Involved Pathway
Dhfr involved in several pathways and played different roles in them. We selected most pathways Dhfr participated on our site, such as Cell Cycle,Cell Cycle, Mitotic,E2F mediated regulation of DNA replication, which may be useful for your reference. Also, other proteins which involved in the same pathway with Dhfr were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Cell cycle | ANAPC5,DPF1,KAT5,CEP78,NEK6,CLASP1A,DCTN1,CENPI,NIPBLB,STAG2 |
| E2F transcription factor network | E2F6,MYBL2,HBP1,YY1,PRMT5,UXT,CDKN2C,RBBP8,TRIM28,CES3 |
| Fluoropyrimidine Activity | SLC22A7,DPYD,DPYS,UPP2,CES1,TYMP |
| Folate biosynthesis | PTS,ALP3,SPRA,FPGS,MOCS1,QDPRB1,GGH,SPR,QDPR,GCH1 |
| G1/S Transition | CKS1B,MYCA |
| Cell Cycle, Mitotic | CSNK2B,LIN52,TSGA14,CLASP1A,CDKN2B,B9D2,MCM10,GORASP2,LMNB1,CEP70 |
Protein Function
Dhfr has several biochemical functions, for example, NADPH binding,dihydrofolate reductase activity,drug binding. Some of the functions are cooperated with other proteins, some of the functions could acted by Dhfr itself. We selected most functions Dhfr had, and list some proteins which have the same functions with Dhfr. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| drug binding | PDE2A,P2RX2,DRD2,CHRNA2,TOP2A,GLRB,PYGL,FNTB,ADRB2,PIGC |
| methotrexate binding | FOLR2,FOLR1,DAAM2 |
| mRNA binding | RBFOX1L,RBM5,SNRNP35,RBMY1A1,C1QBP,KIAA0020,RPS13,RBM25,RBM8A,SRSF1 |
| folic acid binding | DMGDH,FOLR2,SLC19A1,SLC46A1,GNMT,DAAM2,TYMS,FOLR1,FOLR3,FTCD |
| dihydrofolate reductase activity | DHFRL1 |
| NADPH binding | FASN,MTRR,QDPR,CRYZ,DECR1,HMGCR,CBR4,TP53I3,KCNAB1,GRHPR |
Interacting Protein
Dhfr has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Dhfr here. Most of them are supplied by our site. Hope this information will be useful for your research of Dhfr.
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References
- Ershler, MA; Olovnikova, NI; et al. Production of IgM monoclonal antibodies in DG44 cells. APPLIED BIOCHEMISTRY AND MICROBIOLOGY 50:819-828(2014).
- Nasseri, SS; Ghaffari, N; et al. Increased CHO cell fed-batch monoclonal antibody production using the autophagy inhibitor 3-MA or gradually increasing osmolality. BIOCHEMICAL ENGINEERING JOURNAL 91:37-45(2014).
