Recombinant Mouse Ddx20 Protein, Myc/DDK-tagged

• Cat.No.: Ddx20-2501M
• Product Overview: Purified recombinant protein of mouse full-length DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
• Molecular Mass: 92.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ddx20 DEAD box helicase 20 [ Mus musculus (house mouse) ]
• Official Symbol: Ddx20
• Synonyms: DDX20; DEAD (Asp-Glu-Ala-Asp) box polypeptide 20; probable ATP-dependent RNA helicase DDX20; ROSF-1; gemin-3; DEAD box protein 20; component of gems 3; DEAD box protein DP 103; regulator of steroidogenic factor 1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20; dp103; GEMIN3; MGC159174
• Gene ID: 53975
• mRNA Refseq: NM_017397
• Protein Refseq: NP_059093
• UniProt ID: Q9JJY4