Recombinant Mouse Dcps Protein, Myc/DDK-tagged

• Cat.No.: Dcps-2478M
• Product Overview: Purified recombinant protein of mouse full-length decapping enzyme, scavenger (Dcps), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP. May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP. Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 39 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dcps decapping enzyme, scavenger [ Mus musculus (house mouse) ]
• Official Symbol: Dcps
• Synonyms: DCPS; decapping enzyme, scavenger; scavenger mRNA-decapping enzyme DcpS; DCS-1; HINT-5; mRNA decapping enzyme; histidine triad protein member 5; hint-related 7meGMP-directed hydrolase; AA408441; 1700001E16Rik
• Gene ID: 69305
• mRNA Refseq: NM_027030
• Protein Refseq: NP_081306
• UniProt ID: Q9DAR7