Recombinant Mouse Dclk1 Protein, Myc/DDK-tagged

• Cat.No.: Dclk1-2473M
• Product Overview: Purified recombinant protein of mouse full-length doublecortin-like kinase 1 (Dclk1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the biological validity of some variants has not been determined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 84.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dclk1 doublecortin-like kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Dclk1
• Synonyms: DCLK1; doublecortin-like kinase 1; serine/threonine-protein kinase DCLK1; CLICK-I beta; doublecortin-like and CAM kinase-like 1; doublecortin and calcium/calmodulin-dependent protein kinase-like 1; Dcl; Dclk; Cpg16; Click-I; Dcamkl1; AI836758; mKIAA0369; 1700113D08Rik; 2810480F11Rik
• Gene ID: 13175
• mRNA Refseq: NM_019978
• Protein Refseq: NP_064362
• UniProt ID: Q9JLM8