Recombinant Mouse Ctsz protein(Met1-Val306), His-tagged

• Cat.No.: Ctsz-283M
• Product Overview: Recombinant Mouse CTSZ (NP_071720.1)(Met 1-Val 306) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-306 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is >1, 200 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse CTSD pro form consists of 295 amino acids and has a predicted molecular mass of 33.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCTSD is approximately 38 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ctsz cathepsin Z precursor [ Mus musculus ]
• Official Symbol: Ctsz
• Synonyms: CTSZ; cathepsin Z precursor
• Gene ID: 50812