Recombinant Mouse Crh protein, His-tagged

• Cat.No.: Crh-7792M
• Product Overview: Recombinant Mouse Crh aa. (Arg28~Arg160) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Arg28~Arg160
• Description: This gene encodes a member of the corticotropin-releasing factor family and preproprotein that is proteolytically processed to generate a mature protein product. This protein product is a neuropeptide hormone that binds to the corticotropin releasing hormone receptors (CRHR1 and CRHR2) to stimulate the release of adrenocorticotropic hormone from the pituitary gland in response to stress. The encoded protein may also regulate angiogenesis and inflammation. Homozygous knockout mice for this gene exhibit reduced corticosterone levels while the offspring of these mice die perinatally.
• Form: Freeze-dried powder
• Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 10.3.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Crh corticotropin releasing hormone [ Mus musculus (house mouse) ]
• Official Symbol: Crh
• Synonyms: Crh; corticotropin releasing hormone; CRF; Gm1347; corticoliberin
• Gene ID: 12918
• mRNA Refseq: NM_205769.3
• Protein Refseq: NP_991338.1
• UniProt ID: Q8CIT0