Recombinant Mouse Col3a1 protein, His-tagged

• Cat.No.: Col3a1-7928M
• Product Overview: Recombinant Mouse Col3a1 aa. (Gly434~Pro643 (Accession # P08121)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes the alpha-1 subunit of the fibril-forming type III collagen found in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. The encoded protein forms homotrimeric type III procollagen that undergoes proteolytic processing during fibril formation. A majority of mice lacking the encoded protein die within two days of birth but about 5% of the animals survive to adulthood. The surviving mice exhibit severe cortical malformation and experience significantly shorter lifespan. The mutant mouse named "tight skin 2" exhibiting systemic sclerosis phenotype was found to harbor a missense point mutation in this gene. A pseudogene of this gene has been defined on chromosome 8.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.3kDa
• Protein length: Gly434~Pro643
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.1.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Col3a1 collagen, type III, alpha 1 [ Mus musculus (house mouse) ]
• Official Symbol: Col3a1
• Synonyms: Col3a1; collagen, type III, alpha 1; Tsk2; Tsk-2; Col3a-1; AW550625; collagen alpha-1(III) chain; procollagen, type III, alpha 1
• Gene ID: 12825
• mRNA Refseq: NM_009930.2
• Protein Refseq: NP_034060.2
• UniProt ID: P08121