Native Bovine COL3A1 Protein

• Cat.No.: COL3A1-17B
• Product Overview: Purified Bovine Collagen Type III (Atelocollagen) from bovine placenta

Specification

• Description: Type III collagen is the second most abundant collagen in tissues and is found most commonly in tissues exhibiting elastic properties such as skin, lungs, intestinal walls and walls of blood vessels. It is a homotrimer comprised of three alpha-1 chains and resembles other fibrillar collagens in structure and function. It is synthesized as procollagen, similary to collagen I, but the N-terminal propeptide remains attached in the mature fibrillar type III form.
  Mutations of type III collagen causes Ehlers-Danlos syndrome, EDS IV, which affect arteries, internal organs, joints and skin, and may cause sudden death when the large arteries rupture.
• Source: Bovine placenta
• Species: Bovine
• Form: Lyophilized, salt free, 10 mg/vial
• Purity: Bovine Collagen Type III (Atelocollagen) > 90%
  Bovine Collagen Type I (Atelocollagen) < 10%
• Applications: Coating material for cell culture studies or 3D gel formation.
• Storage: Bovine Collagen Type III (Atelocollagen) dissolved in acetic acid is stable at 4 centigrade for 1 month. Lyophilized collagen long term storage (2 years) at -20 centigrade or lower.
• Reconstitution: Dissolve in 10-50 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8 centigrade. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml.

Gene Information

• Official Symbol: COL3A1
• Synonyms: COL3A1; Collagen type III, alpha 1; Collagen III, alpha-1 polypeptide; collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant); EDS4A; Collagen Type III