Recombinant Mouse Cnot3 Protein, Myc/DDK-tagged

• Cat.No.: Cnot3-2220M
• Product Overview: Purified recombinant protein of mouse full-length CCR4-NOT transcription complex, subunit 3 (Cnot3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 82.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cnot3 CCR4-NOT transcription complex, subunit 3 [ Mus musculus (house mouse) ]
• Official Symbol: Cnot3
• Synonyms: CNOT3; CCR4-NOT transcription complex, subunit 3; CCR4-NOT transcription complex subunit 3; CCR4-associated factor 3; A930039N10Rik; MGC40675
• Gene ID: 232791
• mRNA Refseq: NM_146176
• Protein Refseq: NP_666288
• UniProt ID: Q8K0V4